An antiviral T-Cell clone defines a functional supertypic specificity shared by different HLA-DR molecules fromDR2-short,DRw11, andDRwl3Haplotypes
✍ Scribed by Dominique Zeliszewski; Ghislaine Sterkers; Jeanine Choppin; Catherine Freidel; Lucette Gebuhrer; Hervé Betuel; Jean Paul Levy
- Publisher
- Springer-Verlag
- Year
- 1987
- Tongue
- English
- Weight
- 697 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0093-7711
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✦ Synopsis
An influenza virus-specific HLA class IIrestricted human T4 + clone (Ij) allows us to define a new functional supertypic HLA class II specificity shared by three different haplotypes. Influenza A virus-infected antigen-presenting cells of these three haplotypes, HLA-DR2 short, DRw11, and DRw13, are able to stimulate Ij cells. The same precise viral specificity is seen in all three cases. Proliferation inhibition experiments using HLAspecific monoclonal antibodies demonstrate that HLA-DR products are involved in all cases. However, according to the DR specificity of the antigen-presenting cell, differential blockings by a series of DR-specific monoclonal antibodies suggest that the functional epitope is shared by different HLA-DR molecules. This is confirmed by twodimensional gel analysis of the HLA-DR~ chains expressed in the three haplotypes.