An Analyte Derivatization Approach for Improved Electrochemical Detection of Amoxicillin

✍ Scribed by Mei-Hsin Chiu; Jen-Lin Chang; Jyh-Myng Zen

• Book ID: 102182656
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 200 KB
• Volume: 21
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200904604

✦ Synopsis

Abstract

This paper introduces an interesting analyte derivatization approach for improved electrochemical detection of amoxicillin (one of the most frequently used β‐lactam antibiotics). It is based on the well‐known chemical conversion of catechols to o‐quinones by MnO~2~ that can be subsequently easily determined by electrochemical reduction at inexpensive screen‐printed carbon electrodes. The MnO~2~ reactor is essential to the proposed amperometric sensor not only to increase the electroactivity but also to achieve the detection selectivity. Under the optimized conditions of applied potential=0.0 V vs. Ag/AgCl and flow rate=500 μL/min, the measurement range was over 3 orders of magnitude with a detection limit (S/N=3) of 17 nM.