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An analysis of the results for the binding of formaldehyde to polyuridylic acid

✍ Scribed by N. N. Aylward

Publisher: Wiley (John Wiley & Sons)
Year: 1972
Tongue: English
Weight: 357 KB
Volume: 11
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1972.360110705

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✦ Synopsis

Spectrophotometric measurements were made on the extent of binding of formaldehyde to polyuridylic acid under conditions of varying temperature and formaldehyde concentration. The data is interpreted in terms of a temperature-dependent stacking of the bases in poly U at 20°, but not at 40°C. A theory of cooperative stacking is developed which considers the base residues to be either non-bonded,, non-bonded and methylolated, or stacked. The results indicate essentially non-cooperative base stacking under these conditions with an equilibrium constant for base stacking of 0.92 at 20°C.

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