An analysis of the hemoglobins from some common turtles
β Scribed by Horton, Bennett ;Fraser, Ronald ;Dupourque, Daniel ;Bailey, David ;Chernoff, Amoz
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- English
- Weight
- 839 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0022-104X
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β¦ Synopsis
A chromatographic analysis of the blood from seven species of turtles, representing four families, has revealed that all animals contained muItiple hemoglobins. Members of the Emydidae family had two major components separated by a minor one. There was some variation to this pattern in the box turtles (Terrapenes). The musk, soft shelled, and the snapping turtles examined had three, five, and four hemoglobins respectively. In general, electrophoresis of these proteins in starch gels confirmed the results obtained from DEAE cellulose chromatography in minicolumns.
Hemoglobins from the painted and Cumberland turtles were found to be quite resistant to dimerization on aging and to alkali denaturation. These proteins from the box turtles were seen to be moderately resistant to both types of molecular modification, while those from the musk, the soft shelled, and the snapping turtles polymerized readily on aging and denatured readily in alkali.
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