An Amperometric Immunosensor for IgG Based on Conducting Polymer and Carbon Nanotube-Linked Hydrazine Label

✍ Scribed by Ye Zhu; Wei Choon Alvin Koh; Yoon-Bo Shim

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 351 KB
• Volume: 22
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.201000394

✦ Synopsis

Abstract

An amperometric immunosensor for IgG was developed by covalently immobilizing anti‐IgG on multiwall carbon nanotube‐embedded conducting polymer, poly‐5,2′ : 5′′,2′′‐terthiophene‐3′‐carboxylic acid (MWCNT/pTTCA). The MWCNT/pTTCA modified electrode was characterized by SEM, EIS, and XPS. A hydrazine‐labeled secondary antibody‐MWCNT conjugate (Hyd‐MWCNT‐Ab~2~) was applied for detection. Hydrazine was used as a catalyst for the reduction of hydrogen peroxide, which was monitored at −0.3 V vs. Ag/AgCl. The calibration plots showed a linear range of 0.1–10 ng/mL with a detection limit of 0.084±0.004 ng/mL. The proposed immunosensor was evaluated for clinical applications in a rabbit serum sample.