✦ LIBER ✦
An Amperometric Cellobiose Dehydrogenase-Based Biosensor Can Be Used for Measurement of Cellulase Activity
✍ Scribed by Lars Hildén; Lars Eng; Gunnar Johansson; Sten-Eric Lindqvist; Göran Pettersson
- Book ID
- 102566608
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 117 KB
- Volume
- 290
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision.