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An alternatively spliced transcript of the rat nociceptin receptor ORL1 gene encodes a truncated receptor

✍ Scribed by Guo-xi Xie; Emi Ito; Kazuo Maruyama; Christian Pietruck; Manohar Sharma; Long-Chuan Yu; Pamela Pierce Palmer

Book ID
117385467
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
977 KB
Volume
77
Category
Article
ISSN
0169-328X

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Using the combination of a cDNA library prepared from membrane glucocorticoid (mGR)-enriched S-49 cells and a mouse leukocyte genomic library, we have cloned a 7.3 kb full-length glucocorticoid receptor 1A cDNA. Primer extension, 5ЈRACE, and long distance PCR identified the transcription start site