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An Alternative Explanation for the Catalytic Proficiency of Orotidine 5‘-Phosphate Decarboxylase

✍ Scribed by Lee, Tai-Sung; Chong, Lillian T.; Chodera, John D.; Kollman, Peter A.


Book ID
126948527
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
153 KB
Volume
123
Category
Article
ISSN
0002-7863

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A gene bank of Sau3A partially digested Candida albicans DNA in vector YEp13 was used to complement a ura3 mutation (orotidine-5'-phosphate decarboxylase, OMPdecase) in S. cerevisiae. Two plasmids which complemented ura3 and showed clear linkage of Ura+ and plasmid markers were selected for further