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An alternative analysis of enzyme systems based on the whole reaction time: evaluation of the kinetic parameters and initial enzyme concentration

✍ Scribed by R. Varón; M. Garcia-Moreno; J. Masiá-Pérez; F. García-Molina; F. García-Cánovas; E. Arias; E. Arribas; F. García-Sevilla


Publisher
Springer
Year
2006
Tongue
English
Weight
434 KB
Volume
42
Category
Article
ISSN
0259-9791

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A special mixing device for initiating enzyme-catalyzed reactions is used to rapidly achieve an unperturbed quasi-steady state. An on-line computer is employed to sample the initial conditions, the mixing time, and concentrations that change as a function of time during this quasi-steady state phase