An agarose derivative containing an arsenical for affinity chromatography of thiol compounds

✍ Scribed by Ulf Hannestad; Per Lundqvist; Bo Sörbo

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 398 KB
• Volume: 126
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90129-4

✦ Synopsis

An adsorbent for affinity chromatography of thiol compounds was prepared by covalently linking paminophenylarsine oxide to CNBr-activated Sepharose 6B. The adsorbent retained mono-and dithiols from acid and neutral solutions. Monothiols and 1 ,Cdithiols were eluted at pH 12, whereas more alkaline conditions were necessary for elution of 1,2-and 1,3dithiols. The latter could also be eluted at pH 12 by 0.01 M phenylarsine oxide. Alternatively, thiol compounds may be eluted at neutral or acid conditions by other thiol compounds. Monothiols and 1,4dithiols were thus eluted by cysteine, whereas 1,2-and 1,3dithiols required dithiopropylamine for elution.