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An affordable high-performance pumping system for gradient capillary liquid chromatography

✍ Scribed by T. Scott Kephart; Purnendu K. Dasgupta; James N. Alexander IV

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 111 KB
Volume: 11
Category: Article
ISSN: 1040-7685
DOI: 10.1002/(sici)1520-667x(1999)11:4<299::aid-mcs7>3.0.co;2-1

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✦ Synopsis

This article describes a portable capillary-scale liquid delivery system capable of reproducible gradients at constant microliter-per-minute-scale flow rates based on two independent syringe pumps controlled by custom software. The simple-to-build stainless steel syringe pumps are capable of operation at pressures exceeding 8000 psi. System performance is demonstrated using three capillaries packed in house. Reproducibility of the chromatographic system and column efficiencies are reported.

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