An advanced image analysis system for evaluation of somatic embryo development

✍ Scribed by Chung-Ming Chi; Chun Zhang; E. John Staba; Todd J. Cooke; Wei-Shou Hu

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 821 KB
• Volume: 50
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19960405)50:1<65::aid-bit8>3.0.co;2-#

✦ Synopsis

Somatic embryogenesis is among the most promising means of large scale plant micropropagation. The development of somatic embryos ischaracterized by their morphological changes. Embryos in culture usually exhibit high heterogeneity and abnormality. As conventional microscopic observation is laborious and subjective, an objective and quantitative rnorphokinetic description is importantforfurther advancement of this important process technology. We developed an image analysis system capable of measuring morphological and size features of embryos. Subtle environmental effects on embryo development, which are often masked by the subjectivity of microscopic observation, are now discernible by statistically comparing the distributions of these morphological and size features. This image analysis and pattern recognition system was applied t o examine the kinetics of a fed-batch culture. 0 1996