✦ LIBER ✦
An active-site titrant for chymotrypsin, and evidence that azapeptide esters are less susceptible to nucleophilic attack than ordinary esters
✍ Scribed by Jill M. Gassman; Joe Magrath
- Book ID
- 103983477
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 187 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0960-894X
No coin nor oath required. For personal study only.
✦ Synopsis
The p-nitrophenyl ester of N-methoxycarbonylazaphenylalanine reacts with chymotrypsin in 1:1 stoichiometry, and the enzyme concentration can be quantified from the absorbance of the p-nitrophenolate produced in the reaction. This azapeptide does not decompose at pH 7 in the presence or absence of 1 mM hydroxylamine.