𝔖 Bobbio Scriptorium
✦   LIBER   ✦

An active insoluble aggregate of E. coli β-galactosidase

✍ Scribed by S. K. Khare; M. N. Gupta


Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
335 KB
Volume
35
Category
Article
ISSN
0006-3592

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Complementary DNAs encoding mature ␤-glucosidase proteins Glu1 and Glu2 of maize were amplified by the polymerase chain reaction (PCR) and cloned into the expression vector pET21a. Both Glu1 and Glu2 isozymes were expressed in high yield (∼3.8% of the total soluble protein and 32% of the total expre