Amplified Fluorescence Turn-On Assay for Mercury(II) Detection and Quantification based on Conjugated Polymer and Silica Nanoparticles

✍ Scribed by Yusong Wang; Bin Liu

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 317 KB
• Volume: 30
• Category: Article
• ISSN: 1022-1336
• DOI: 10.1002/marc.200800727

✦ Synopsis

Abstract

An amplified fluorescence turn‐on assay for mercury(II) detection and quantification was developed. This method makes use of specific thymine/mercury(II)/thymine coordination to capture Fl‐labeled DNA onto NP surface. Addition of a cationic conjugated polymer leads to an amplified Fl signal in solution. A sigmoidal Hg^2+^ working curve is obtained at fixed [NP] with a detection limit of 0.1 × 10^−6^ M. However, by reducing [Hg^2+^] and [NP] simultaneously, while maintaining [Hg^2+^]:[DNA duplex] = 3:1, a linear calibration curve is observed with a detection limit of 5 × 10^−9^ M. The CCP‐assisted mercury(II) assay shows potential applications in environmental mercury detection and for industrial process control.

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