Amplification of immunoglobulin λ constant genes in populations of wild mice
✍ Scribed by Scott, Cynthia L.; Mushinski, J. Frederic; Huppi, Konrad; Weigert, Martin; Potter, Michael
- Book ID
- 109729481
- Publisher
- Nature Publishing Group
- Year
- 1982
- Tongue
- English
- Weight
- 498 KB
- Volume
- 300
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/300757a0
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📜 SIMILAR VOLUMES
Genomic DNA for the immunoglobulin (Ig) constant kappa Igk-C gene region was amplified by the polymerase chain reaction (PCR) method and sequenced from twelve commonly used inbred mouse strains. PCR products were used directly as templates in dideoxy-DNA-sequencing, a method which avoids the sequenc
H-2b-restricted cytolytic T lymphocytes (CTL) were generated against H-1, H-3, and H-4 antigens and tested against target cells of F1 hybrids between wild mice and inbred H-2b mice. The congenic strain combinations for the CTL production were such that they tested one allele each at the H-1 and H-4