Amplification of HMG-CoA Reductase Production Enhances Carotenoid Accumulation in Neurospora crassa
✍ Scribed by Guang-Yi Wang; Jay D. Keasling
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 508 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1096-7176
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✦ Synopsis
Neurospora crassa, a filamentous fungus, naturally produces the carotenoids lycopene and neurosporaxanthin. To increase the carbon flux through the carotenoid biosynthetic pathway, the 1658-bp region of the HMG1 gene encoding the catalytic domain (cHMG1) of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase of Saccharomyces cerevisiae was expressed in N. crassa under control of the strong, constitutive glyceraldehyde-3-phosphate dehydrogenase (GPD) promoter and the inducible alcohol dehydrogenase (alcA) promoter. Overexpressing cHMG1 under control of the GPD promoter increased lycopene and neurosporaxanthin production 6- and 1.5-fold, respectively, relative to the wild-type strain. Over-expression of cHMG1 under control of the alcA promoter increased production of lycopene and neurosporaxanthin 3- and 2-fold, respectively.