Amphotericin B toxicity as related to the formation of oxidatively modified low-density lipoproteins
✍ Scribed by Joanna Barwicz; Isabelle Dumont; Claire Ouellet; Ilona Gruda
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 518 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1075-4261
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✦ Synopsis
The effect of amphotericin B on the oxidation and degradation of low- and high-density lipoproteins was investigated by UV-vis spectroscopy, electron microscopy, electrophoresis, and size-exclusion chromatography. Two formulations of the drug were used: the commercial Fungizone and a new, less toxic, liposomal formulation, AmBisome. It was shown that Fungizone strongly enhanced the oxidative deformation of low-density lipoprotein structure while AmBisome did not bind to this lipoprotein fraction and did not affect its oxidation. It was shown that amphotericin B contained in Fungizone extracted cholesterol from low-density lipoproteins which sensitized them to oxidation. Both formulations of amphotericin B studied here did not bind to high-density lipoprotein and did not affect the process of its oxidation.