Amperometric needle-type glucose sensor based on a modified platinum electrode with diminished response to interfering materials
✍ Scribed by Chien-Yuan Chen; Masao Gotoh; Hideichi Makino; Yuan-Chi Su; Eiichi Tamiya; Isao Karube
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 886 KB
- Volume
- 265
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
A needle-type glucose sensor that can be used to determine glucose in serum and whole blood samples was developed Platmum wre was used as the workmg electrode and a disposable hypodernuc stamless-steel needle electroplated with platinum was used as the counter and reference electrode A method mvolvmg both photocrosslinking of PVA-SbQ and cross-Mung with glutaraldehyde was used to unmobdize the enzyme [PVA-SbQ IS a poly(vmyl alcohol) bearing stdbazohum groups] Nafion and cellulose triacetate membranes were used to prevent inaccuracy from mterfenng materials and to increase the dynanuc range of the sensor, respectwely The response, reproducdxhty and long-term stablhty of the sensor and the effects of temperature, pH and metal Ions on the response were investigated Qwmg to the effective method for enzyme unmolxhzat~on, the large surface area of the counter electrode and the relative mactwity of the counter electrode to chemical reactions, the sensor showed good response, statuhty and reproducMlty
The sensor did not respond to ascorbate and urate at the concentrations normally found m blood Data obtained from the sensor for glucose m serum and whole blood samples showed a good correlation (r > 0 95) with a chmcal laboratory automated analyser