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Amperometric determination of sulfite with sulfite oxidase immobilized at a platinum electrode surface

✍ Scribed by Tekum Fonong

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 261 KB
Volume: 184
Category: Article
ISSN: 0003-2670
DOI: 10.1016/s0003-2670(00)86493-4

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✦ Synopsis

Sulfite oxidase is immobilized on collagen membrane at the surface of a platinum electrode and catalyzes the oxidation of sulfite to sulfate with stoichiometric production of hydrogen peroxide. The hydrogen peroxide is detected amperometically at the platinum electrode at an applied potential of 700 mV. The system responds linearly to sulfite in the range l-150 PM, with a detection limit of 0.2 PM. The enzyme retains over 95% of its activity for three weeks if stored at -20°C when the probe is not in use.

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