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Amperometric determination of phosphate by use of a nucleoside phosphorylase-xanthine oxidase enzyme sensor based on a clark-type hydrogen peroxide or oxygen electrode

✍ Scribed by Stephan D. Haemmerli; Ahmad A. Suleiman; George G. Guilbault


Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
409 KB
Volume
191
Category
Article
ISSN
0003-2697

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✦ Synopsis


A bienzymatic sensor for the determination of phosphate was constructed by coimmobilization of xanthine oxidase (EC 1.2.3.22) and nucleoside phosphorylase (EC 2.4.2.1) on a polycarbonate membrane mounted on the tips of amperometric hydrogen peroxide and oxygen electrodes. The sensor response was linear to phosphate concentrations in the range 10-250 microM.