Amperometric Detection of Glucose with Glucose Oxidase Absorbed on Porous Nanocrystalline TiO2 Film

✍ Scribed by Qingwen Li; Guoan Luo; Jun Feng; Qun Zhou; Li Zhang; Yongfa Zhu

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 107 KB
• Volume: 13
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/1521-4109(200104)13:5<413::aid-elan413>3.0.co;2-i

✦ Synopsis

Porous nanocrystalline TiO 2 may be a promising material for enzyme immobilization owing to its high biocompatiability and large speci®c surface area of nanosized particles. We exploited a novel route to synthesize nanosized TiO 2 particles with TiCl 4 as precursor. With this method, high purity of the oxide product can be obtained and the resulted particles were in the size range at about 5 nm. The enzyme loading with such material was 13 % (waw, GODaTiO 2 ). The interaction between the enzyme and TiO 2 matrix was investigated by IR and Raman, as the main characteristic peaks of native GOD could be found in TiO 2 -GOD at almost the same positions, it implied that the immobilization process did little harm to the structure of GOD. The nanocrystalline TiO 2 -GOD based sensor was fabricated. Amperometric detection of glucose with such an unmediated sensor at 0.7 V (vs. SCE) resulted in a rapid, stable and linear response in the concentration range of 0±3 mM with apparent Michaelis-Menten constant K app M of 6.08 mM, indicating that the immobilized GOD on nanosized TiO 2 matrix retained its native activity.