Amperometric and colorimetric enzyme immunoassay for urinary human serum albumin using a plasma-treated membrane
✍ Scribed by Shigeki Kaku; Setsuko Nakanishi; Kazuo Horiguchi; Masanori Sato
- Book ID
- 102982244
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 630 KB
- Volume
- 281
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
By water vapour plasma glow discharge treatmentƘ microporous hydrophobic polypropylene film was modified to make it partially hydrophilic . The plasma-treated part was a spot 6 mm in diameter and permeable to water . After treatment with octamethylenediamine and glutaraldehydeƘ the plasma spot membrane served as a substrate to immobilize proteins such as antibodies or enzymes . Using this membraneƘ an amperometric enzyme immunoassay system was established to determine human serum albumin (HSA) in urine . The measurable range with this system was from 0.5 to 100 mg 1 -1 HSA in urineƘ which is suitable for the prognosis of diabetic nephritis . The HSA values in urine samples determined by the present system showed a linear correlation with those obtained by radioimmunoassay (r = 0 .974). After keeping the antibody-immobilized plasma spot membranes at 4°C for 6 monthsƘ the same calibration graph for HSA determination was obtained . In additionƘ a colour-producing reaction was also performed on the immunoreacted plasma spot membrane . Reflectance spectrometric analysis demonstrated that the colour on the plasma spot membrane itself corresponded to the HSA concentration . The difference in colour between 10 and 100 mg 1-1 HSA could be visually discerned .