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AMP deaminase in rat brain: Localization in neurons and ependymal cells

✍ Scribed by Kerstin Knecht; Karl-Heinz Wiesmüller; Volker Gnau; Günther Jung; Richard Meyermann; Kathryn G. Todd; Bernd Hamprecht

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
668 KB
Volume
66
Category
Article
ISSN
0360-4012

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✦ Synopsis

Abstract

The purine nucleotide cycle enzyme AMP deaminase (AMPD) catalyzes the irreversible hydrolytic deamination of AMP. The physiological function of the purine nucleotide cycle in the brain is unknown. In situ hybridization and immunocytochemical studies were performed to identify the regional and cellular expression of AMPD in rat brain with the goal of elucidating the neural function of the purine nucleotide cycle. AMPD messenger RNA was detected in ventricular ependymal cells and cells of the choroid plexus and in neurons of distinct brain areas. Although only low antibody titers were obtained by immunization with the purified sheep brain AMPD, immunization of mice with synthetic lipopeptide vaccines containing oligopeptides derived from a known partial complementary DNA sequence of the enzyme yielded an antiserum suitable for immunocytochemistry. Immunostaining of cells in culture showed that neurons but not astroglial cells express appreciable amounts of the enzyme. Results of immunocytochemical staining performed on rat brain slices were in accord with the localization of AMPD messenger RNA, thus confirming the expression of AMPD in neurons of the brain stem, hippocampus, cerebellar nuclei and mesencephalic nuclei, as well as in ventricular ependymal cells and their cilia. © 2001 Wiley‐Liss, Inc.

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