✦ LIBER ✦

Ammonia-functionalized particulate poly(glycidyl methacrylate-co-ethylene dimethacrylate)-based polymers as stationary phase for protein retention

✍ Scribed by B Paredes; E Suárez; M Rendueles; M A Villa-García; J M Díaz

Publisher: Wiley (John Wiley & Sons)
Year: 2001
Tongue: English
Weight: 273 KB
Volume: 76
Category: Article
ISSN: 0268-2575
DOI: 10.1002/jctb.503

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✦ Synopsis

Abstract

A poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) polymeric matrix was synthesized, amino‐functionalized and packed to be tested for protein retention and separation. Functionalization was carried out by reacting the epoxy groups with 30% ammonia solution to provide amino groups for anion exchange with proteins. Physical characterization of the particulate material showed that the functionalized polymer is macroporous and exhibits a unimodal pore size distribution. The resin presents good thermal stability. Chromatographic characterization using bovine serum albumin (BSA) and α‐lactalbumin as model proteins showed good retention properties for the amino‐functionalized matrix. The values obtained for the equilibrium constant (K) were around 3 for BSA, both in batch reactor and column‐packed operation mode. The K values for α‐lactalbumin were even higher, 8.01 and 4.65 for the batch and column‐packed experiments, respectively. Finally, axial dispersion coefficients were obtained, with a constant value of 0.0141 cm^2^s^−1^ for BSA. Higher values were obtained for α‐lactalbumin, but in this case the axial dispersion coefficient was not constant and its values were dependent on the flows used.