Amino acid substitutions at position 38 of the DRβ polypeptide confer susceptibility to and protection from primary sclerosing cholangitis
✍ Scribed by J. Mark Farrant; Derek G. Doherty; Peter T. Donaldson; Robert W. Vaughan; Karen M. Hayllar; Ken I. Welsh; Adrian L. W. F. Eddleston; Roger Williams
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 669 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
Previous studies based on serological HLA phenotyping have implicated genes in the HLA class 11 region in susceptibility to and protection from primary sclerosing cholangitis. In a recent report, the HLA DRw52a antigen was present in all 29 patients who had been referred for liver transplantation. In this study, HLA DRB, DQA and DQB genotypes were studied using gene ampli6cation and sequence-specific oligonucleotide probing in 71 patients with primary sclerosing cholangitis and 68 healthy controls to determine the frequency among the patients of the DRB3*0101 allele that encodes DRw52a and whether other class II alleles are involved in susceptibility or protection. DRB3*0101 was the most strongly associated allele, being present in 55% of the patients and 22% of the controls. Survival among the DRB3*0101-positive patients was reduced compared with the DRB3*0101-negative patients. Both DRB3*0101 and DRB6*0101, a possible second DRB susceptibility allele, encode a leucine residue at position 38 of the DRf3 molecule. The DRB4*0101 allele, which encodes DRw63 and may be protective, encodes an alanine residue at this position. Susceptibility to and protection from primary sclerosing cholangitis may result from amino acid substitutions at position 38 of the DRB molecule because maximum relative risk was conferred by two leucine-38-containing DRB molecules, whereas minimum relative risk was conferred by two alanine-38-containing molecules. (HEPATOLOGY 1992; 16:390-395.)
Overrepresentation of the HLA antigens B8 and DR3 among patients with primary sclerosing cholangitis (PSC) was reported almost 10 yr ago (1-31, and in a recent study of 81 patients from this Institute these