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Amino acid sequencing by gas chromatography-mass spectrometry using perfluoro-dideuteroalkylated peptide derivatives: B. Interpretation of the mass spectra

✍ Scribed by H. Nau; K. Biemann

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 955 KB
Volume: 73
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90151-2

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✦ Synopsis

The mass spectra of the 0-trimethylsilylated trifluoro-dideuteroethyl polyamino alcohols, produced by LiAID,-reduction and 0-trimethylsilylation of Ntrifluororacetyl oligopeptide methyl esters, are evaluated. Characteristic mass spectra of derivatives are shown which are derived from peptides containing all protein amino acids including Arg, His, Trp, Gin, Asn and carboxyl terminal amides as well as modified Cys-residues. The mass spectra of these derivatives can be easily interpreted in terms of the amino acid sequence of the original peptides since they contain abundant and intensity-balanced sequencedetermining ions.

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