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Amino-Acid Sequence of the Vitamin-K-Dependent Part of Protein Z

✍ Scribed by Peter HØJRUP; Peter ROEPSTORFF; Torben E. PETERSEN


Book ID
115121279
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
554 KB
Volume
126
Category
Article
ISSN
1432-1327

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We report the first direct method for the identification of the vitamin K-dependent Ca2+ binding amino acid, gamma-carboxyglutamic acid (Gla), in the sequencing of proteins. The carboxyl groups on the protein are first converted to methyl esters with methanolic HCl, a procedure that reduces the pola