Amino acid sequence of a basic aspartate-49-phospholipase A2 from Trimeresurus flavoviridis venom and phylogenetic analysis of Crotalinae venom phospholipases A2
β Scribed by Takahito Chijiwa; Kazuki Abe; Tomohisa Ogawa; Nikolai N. Nikandrov; Shosaku Hattori; Naoko Oda-Ueda; Motonori Ohno
- Book ID
- 116921384
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 381 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0041-0101
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Trimeresurus fivoviridis (Habu snake) venom aspartate-49-phospholipase A2 (Asp-49-PLAz) was reacted at pH 9.0 with a 2-fold molar excess of 2,4,6-trinitrobenzenesdfonate in the absence of Ca" and two trinitrophenylated derivatives were isolated by HPLC. One was a derivative modified at Lys-11 and it
Basic proteins I and I1 (BP-I and BP-11) isolated from Tnmeresurusflavoviridis venom, which are classified into a group of lysine-49-phospholipases A2 (Lys-49-PLA3, exhibited only limited lipolytic activity for the mixed micelles of various phospholipids. Based on the finding that BP-11 elicits a st