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Amino acid sequence differences in pancreatic ribonucleases from water buffalo breeds from Indonesia and Italy

✍ Scribed by Abubakar Sidik; Bert Martena; Jaap J. Beintema


Book ID
104788877
Publisher
Springer
Year
1979
Tongue
English
Weight
403 KB
Volume
17
Category
Article
ISSN
0006-2928

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✦ Synopsis


The amino acid sequences of the pancreatic ribonucleases from river-breed water buffaloes from Italy and swamp-breed water buffaloes from Indonesia differ at three positions. One of the differences involves a replacement of asparagine-34, with covalently attached carbohydrate on all molecules, in the river-breed enzyme by serine in the swamp-breed enzyme. The ribonuclease content of the pancreas differs considerably between breeds and is lower in river buffaloes. A ribonuclease preparation from two swamp buffaloes contained a minor glycosylated component. Preliminary evidence was obtained that the amino acid sequence of this component has factors in common with the main component of the swamp-breed ribonuclease and with the river-breed enzyme.