Amino acid incorporation into total protein and levels of S-100 protein in discrete rat brain areas after prolonged protein or amino acid restriction
✍ Scribed by L. Rosengren; A. Wronski; Dr. K. G. Haglid; J. Jarlstedt; L. Rönnbäck
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 533 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Abstract
Young adult rats were fed an isocaloric diet for 28 days. The diet consisted of either 22% casein, or protein‐restricted food consisting of a 5% mixture of soy and whey protein, or a synthetic amino acid mixed diet devoid of the essential amino acids tryptophan, valine, lysine, and threonine.
The level of the nervous tissue‐specific S‐100 protein was quantified immunoelectrophoretically in 9 different brain areas. A marked decreased of S‐100 was observed in hippocampus and posterior part of the cerebellar vermis per g wet weight in protein‐restricted rats. An additional lowering was noticed in sensory motor cortex per mg soluble protein. This effect was potentiated with the amino acid deficient diet.
The in vitro incorporation of ^3^H‐leucine into cerebellar proteins was slightly decreased in the low protein‐fed rats, and more markedly decreased in those receiving an amino acid‐restricted diet.