Amino acid esters of 9-(2′,3′-dihydroxypropyl-1′)-adenine are the specific inhibitors of protein synthesis on ribosomes

✍ Scribed by B. P. Gottikh; A. A. Krayevsky; M. K. Kukhanova; A. A. Jatsyna; A. M. Kritzyn; C. L. Florentiev

• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 292 KB
• Volume: 1
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00357159

✦ Synopsis

Peptide acceptor properties of phenylalanine and glycine esters of 9'-(2',3'-dihydroxypropyl-1')-adenine and 1-(2',3'-dihydroxypropyl-1')-4-thiouracyl were investigated. All these esters appeared to be powerful inhibitors of polyphenylalanine synthesis in E. coli MRE-600 ribosomes charged with poly U. Like puromycin, esters of adenine derivatives accepted the AcPhe residue from Ac-[(14)C] Phe-tRNA in a ribosomal system charged with poly U. However, peptidyl esters of 9-(2',3'-dihydroxypropyl-1')-adenine remained bound with ribosomes. The structure of the peptide esters synthesized was ascertained after dissociation of ribosomes into subparticles by direct comparison with the synthetic specimens.