Amino acid and energy requirements for rat hepatocytes in primary culture
β Scribed by Per E. Schwarze; Anne E. Solheim; Per O. Seglen
- Book ID
- 112895829
- Publisher
- Society for In Vitro Biology
- Year
- 1982
- Tongue
- English
- Weight
- 881 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1475-2689
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## Abstract Transmembrane potential (E~m~) and alphaβaminoisobutyric acid (AIB) transport were measured in primary monolayer cultures of rat hepatocytes obtained from unoperated control rats and from rats 12 hr following partial hepatectomy. Measurements were performed 20β24 hr after plating the ce
System N, a neutral amino acid transport system characterized in rat hepatocytes, shows significant changes in activity during the first 24 hr of primary culture (Weissbach, L., Handlogten, M.E., Christensen, H.N., and Kilberg, M.S. [1982] J. Biol. Chem. 257:12006-12011). Experiments presented in th