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Amino acid analysis on polyvinylidene difluoride membranes

✍ Scribed by G.I. Tous; J.L. Fausnaugh; O. Akinyosoye; H. Lackland; P. Winter-Cash; F.J. Vitorica; S. Stein

Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 587 KB
Volume: 179
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(89)90198-x

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bation, 10 ml 0.04 N NaOH was added. Cells of the control treatment were resuspended in 20 ml 0.02 N NaOH. Results, however, showed that zymolyase treatment did not improve but rather worsened PCR amplification. (4) Amount of NaOH compatible with PCR: Under the present conditions, 0.02 N NaOH could