agent should give rise to a single, stable derivative per An improved method for the quantitative derivatizaamino acid. The derivative should be detectable with tion of amino acids with fluorenylmethyl chlorohigh sensitivity and the reagent itself or its degradaformate (FMOC-Cl) is described. Amino
Amino Acid Analysis by Reverse-Phase High-Performance Liquid Chromatography: Improved Derivatization and Detection Conditions with 9-Fluorenylmethyl Chloroformate
โ Scribed by Ruud A. Bank; Evelien J. Jansen; Bob Beekman; Johan M. te Koppele
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 189 KB
- Volume
- 240
- Category
- Article
- ISSN
- 0003-2697
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## Abstract This paper describes a novel method for the sensitive and selective determination of fudosteine in human plasma. The method involves a derivatization step with 9โfluorenylmethyl chloroformate (FMOCโCl) in borate buffer and detection based on highโperformance liquid chromatographyโelectr
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