Amidino-substituted aromatic heterocycles as probes of the specificity pocket of trypsin-like proteases

Benzamidine as a spectroscopic probe for

Benzamidine as a spectroscopic probe for the primary specificity subsite of trypsin-like serine proteinases

✍ Paolo Ascenzi; Martino Bolognesi; Mario Guarneri; Enea Menegatti; Gino Amiconi 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 435 KB

Formation and dissociation of the benzamidine:beta-trypsin adduct is accompanied by reversible spectral changes in the ultraviolet region (between 230 and 300 nm). The pH-independent difference extinction coefficient of the adduct (benzamidine:beta-trypsin complex minus the free proteinase) is 1.75