Amelioration of oxygen-induced osteoporosis in the in vitro fetal rat tibia with a capacitively coupled electrical field

✍ Scribed by Dr. Carl T. Brighton; Claude E. Nichols III; George A. Arangio

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 993 KB
• Volume: 3
• Category: Article
• ISSN: 0736-0266
• DOI: 10.1002/jor.1100030307

✦ Synopsis

Near-term fetal rat tibiae were grown in M.E.M. EagleiNCTC 135/ 15% newborn calf serum in 5% carbon dioxide and 5 , 10, 21, 35, 60, and 90% oxygen for 3, 7 , 10, and 14 days. Linear growth of the explants, as measured from macrophotographs of the explants at day zero and each of the days above, was greatest in the lower oxygen concentrations and least in the higher oxygen concentrations. Breaking strengths of the tibia1 diaphyses were significantly reduced in those explants grown in 60 and 90% oxygen. When the fetal rat tibiae were grown in 60% oxygen for 7 days and were subjected to a capacitively coupled electrical signal (sine wave, 60 kHz, 10 V peak-to-peak output signal; current density and field in the culture dish calculated to be 5.2 pAl cm2 and 0.32 mV/cm, respectively), the breaking strengths and middiaphyseal widths were statistically significantly greater than control tibiae grown in 60% oxygen alone. It is concluded that an appropriate capacitively coupled electrical field can inhibit an oxygen-induced osteoporosis in an in vitro mammalian long bone model.