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Alternative splicing of the FMR1 gene in human fetal brain neurons

✍ Scribed by Huang, Tao; Li, Lan-Ying; Shen, Yan; Qin, Xue-bin; Pang, Zhi-Ling; Wu, Guan-Yun

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 413 KB
Volume: 64
Category: Article
ISSN: 0148-7299
DOI: 10.1002/(sici)1096-8628(19960809)64:2<252::aid-ajmg3>3.0.co;2-w

No coin nor oath required. For personal study only.

✦ Synopsis

The alternative splicing expression of the FMRl gene was reported in several human and mouse tissues. Five regions of FMRl gene can be alternatively spliced, but the combination of them has not been investigated fully. W e reported here the analysis of alternative splicing pattern of the FMRl gene in cultured fetal human neurons, using a RT-PCR and cloning strategy. Eleven splicing types were cloned and different isoforms were not equally represented. The dominant isoform represents nearly 40%, and the other isoforms were relatively rare. One isoform has a different carboxylterminus. Most of the alternative spliced regions appear hydrophilic; thus, they may locate on the surface of the FMRl protein.

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