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Alternating current polarographic investigation of polysaccharides in DNA

✍ Scribed by B. Malfoy; J.A. Reynaud

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
581 KB
Volume
84
Category
Article
ISSN
0003-2697

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✦ Synopsis

Polysaccharides alone or in the presence of DNA are studied by means of alternating current (ac) polarography. When neutral and basic polysaccharides are used, the polarograms recording the quadratic component of the current display one capacitive peak at -1650 mV (SCE). Acid polysaccharides never show this peak and are desorbed from the electrode at more positive potentials. If dextran is used as a reference, this peak allows the determination of the amount of neutral polysaccharides in solution up to 2 PgIml. The height of this peak has no relation to the ionic strength or pH of the solution within the investigated range. Likewise, the concentration and molecular weight of DNA enclosed in the solution exert no influence upon the peak height. On the other hand, the presence of polysaccharides causes DNA peaks to decrease considerably. ac polarography can, therefore, be regarded as a quick, convenient, and sensitive method for carrying out the titration of polysaccharides alone or mixed with DNA.

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