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Altering the specificity of subtilisin B. Lentus by combining site-directed mutagenesis and chemical modification

โœ Scribed by Per Berglund; Michele R. Stabile; Marvin Gold; J.Bryan Jones; Colin Mitchinson; Richard R. Bott; Thomas P. Graycar


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
288 KB
Volume
6
Category
Article
ISSN
0960-894X

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Four residues in the carboxy-terminal domain of human epidermal growth factor (hEGF), glutamate 40, glutamine 43, arginine 45, and aspartate 46 were targeted for site-directed mutagenesis to evaluate their potential role in epidermal growth factor (EGF) receptor-ligand interaction. One or more mutat