Altered protein-expressing profile in hPNAS4-induced apoptosis in A549 human lung adenocarcinoma cells

✍ Scribed by Lan-Tu Gou; Ai-Ping Tong; Fei Yan; Zhu Yuan; Fei He; Wei Wang; Yan Zhou; Li-Juan Chen; Ming-Hai Tang; Jin-Liang Yang

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 371 KB
• Volume: 108
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.22353

✦ Synopsis

Abstract

Human PNAS4 (h__PNAS4__) is a recently identified pro‐apoptosis gene, which is able to induce apoptosis in A549 human lung adenocarcinoma cells following its overexpression. In this work, we investigated the changes of protein profile in h__PNAS4__‐induced apoptosis in A549 cells through proteomic strategy consisting of two‐dimensional electrophoresis (2‐DE) coupled with MALDI‐Q‐TOF mass spectrometry. A total of 20 different proteins with more than 3.0‐fold change in expression, including 5 up‐regulated and 15 down‐regulated proteins were successfully identified by database search. The mRNA transcription levels of the different proteins were further examined by RT‐PCT. Functional analyses showed these different proteins are involved in diverse biological processes including metabolism, proteolysis, signal transduction, apoptosis, and redox regulation. Two essential apoptosis‐associated protein, annexin A1 and prothymosin alpha, were confirmed by Western blot and showed consistent changes with proteomic detection. Our data provide molecular evidence and possible associated pathway in h__PNAS4__‐induced apoptosis through proteomic strategy, which should be contributed to further investigation on biological function of h__PNAS4__. J. Cell. Biochem. 108: 1211–1219, 2009. © 2009 Wiley‐Liss, Inc.