Altered methionine metabolism in metastatic variants of a human melanoma cell line
β Scribed by Liteplo, R.G.
- Book ID
- 122741932
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 799 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0304-3835
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Our aim was to determine if the selection of human tumor cells with enhanced anchorage-independent growth capacity was associated with alterations in methionine auxotrophy. Cells with an increased ability to form colonies on soft agarose were selected from human melanoma (MeWo) and neuroepithel ioma
Methionine dependence is a metabolic defect found t h u s far only in transformed and malignant cells. The defect is manifested as the inability of cells to grow in media in which methionine (Met) is replaced by its immediate precursor homocysteiiie (Hcy). We have termed this Met-Hcy+ media. We demo
## Abstract Our aim was to identify the biochemical defect responsible for the inability of highly growth autonomous human tumor cells to proliferate in culture medium devoid of methionine, but containing homocysteine and 5βmethyltetrahydrofolic acid. We have adopted the terms βhomocysteineβrespons