Altered adhesion properties and alpha v integrin expression in a cisplatin-resistant human ovarian carcinoma cell line

✍ Scribed by Sylvie Maubant; Séverine Cruet-Hennequart; Laurent Poulain; Franck Carreiras; François Sichel; José Luis; Cathy Staedel; Pascal Gauduchon

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: French
• Weight: 416 KB
• Volume: 97
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.1600

✦ Synopsis

Abstract

In order to elucidate the mechanisms underlying the development of chemoresistance in ovarian cancer, we have previously established the IGROV1‐R10 cisplatin‐resistant cell line by mimicking a clinical protocol of drug administration on IGROV1 human ovarian carcinoma cells. Both IGROV1 and IGROV1‐R10 cells were able to grow as a monolayer and to release cell clusters into the medium. However, IGROV1‐R10 cells exhibited an enhanced capacity to detach from the monolayer as compared to the parental cells. When substrate adhesion was prevented, IGROV1‐R10 cells were able to survive and to proliferate as cell clusters, even at a low cell density, whereas IGROV1 cells massively died. To explore the underlying mechanisms, we have been interested in αv integrins, which have been implicated in some aspects of ovarian cancer biology. Both IGROV1 and IGROV1‐R10 adherent cells expressed αvβ3 integrin. During cell growth, αvβ5 integrin accumulated at the surface of a majority of IGROV1‐R10 cells from the monolayer, whereas only a faint expression of this integrin was observed in a minority of IGROV1 cells. The growth of IGROV1‐R10 cells, but not of IGROV1 cells, was partly inhibited by a specific αvβ5‐blocking antibody suggesting that αvβ5 integrin contributed to IGROV1‐R10 cell proliferation. © 2002 Wiley‐Liss, Inc.