Alterations of the retinoblastoma-related gene RB2/p130 in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in rats

✍ Scribed by Toshifumi Tsujiuchi; Yasutaka Sasaki; Yoichi Konishi; Masahiro Tsutsumi

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 124 KB
• Volume: 35
• Category: Article
• ISSN: 0899-1987
• DOI: 10.1002/mc.10074

✦ Synopsis

Abstract

Alteration of the retinoblastoma‐related gene RB2/p130 was investigated in lung adenocarcinomas induced by N‐nitrosobis(2‐hydroxypropyl)amine (BHP) in male Wistar rats. At 6 wk of age, 21 animals were given 2000 ppm of BHP in their drinking water for 12 wk and then maintained without further treatment until they were killed at the end of week 25. A total of 21 lung adenocarcinomas were obtained, and total RNAs were extracted from each for mutation analysis of RB2/p130 by the reverse transcription–polymerase chain reaction–single‐strand comformation polymorphism approach. No mutations were found in exons 19–22. However, examination of the expression of the RB2/p130 gene by Northern blot analysis showed mRNA levels to be significantly lower than those of normal lung tissues. Western blot analysis showed reduction of the pRb2/p130 protein in all of the adenocarcinomas examined. These results suggest that alteration of the RB2/p130 gene may play important roles in the development of lung adenocarcinomas induced by BHP in rats. © 2002 Wiley‐Liss, Inc.