Alterations in phenotype and cell-surface antigen expression levels of human monocytes: Differential response to in vivo administration of rhm-csf or rhgm-csf
✍ Scribed by Ingrid Schmid; Gayle C. Baldwin; Edwin L. Jacobs; Valentin Isacescu; Negoita Neagos; Janis V. Giorgi; John A. Glaspy
- Book ID
- 102816062
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 757 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
We investigated, via multicolor flow cytometry, the in vivo effects of colony-stimulating factors (CSFs) on cell size, frequencies, and expression of surface antigens on peripheral blood monocytes from melanoma patients treated concurrently with CSFs and tumor-specific monoclonal antibody (mAb) R24. Recombinant human macrophage colony-stimulating factor (rhM-CSF) increased cell size, relative percentages of monocytes, percentages of CD14+, HLA-DQ+, C D l l b+, and CD16+ monocytes, and cell-surface expressions of HLA-DR and C D l l b; rhM-CSF also up-regulated cell-surface expression of CD14 on CD14brigh'CD16-monocytes. Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) increased cell size, percentages of CD14+, HLA-DQ+, and C D l l b+ monocytes, and cell-surface expressions of HLA-DR, HLA-DQ, C D l l b, and CD58. Relative percentages of monocytes and CD16+ cells and cell-surface expression of CD14 on CD14brightCD1 6-monocytes decreased. In addition, monocytes derived from patients treated with rhM-CSF showed functional activity when assayed in vitro for antibody-dependent cellular cytotoxicity (ADCC). During treatment and coincident with increased CD16 expression, monocytes derived from rhM-CSF patients had enhanced levels of cytotoxicity towards melanoma target cells compared to healthy controls and to patients treated with rhGM-CSF.