Alterations in gastrointestinal contents induced by elemental diets
โ Scribed by John R. Stanford; Leona W. Ayers; J. Frederick Cornhill; Larry C. Carey; James W. Kilman; William G. Pace; John S. Vasko
- Book ID
- 102440406
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 157 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0022-4790
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โฆ Synopsis
The effects of elemental diets on selected aspects of the rat colon were studied.
Forty young male Sprague-Dawley rats were divided into 4 diet groups of 10 rats each: Purina Rat Chow (control); Flexical; Precision L-R; and Vivonex. All diets were fed ad lib t o rats housed in pairs in wire-bottom cages. Two weeks after weight stabilization had been achieved all rats were killed and colon contents were collected for culture and short-chain fatty acid analysis on the Perkins-Elsoner 3920 gas chromatograph. Colon fecal butyric/acetic acid ratios of the rats in the 4 groups were: Rat Chow, 2.56; Flexical, 0.28; Precision L-R, 0.1 6; and Vivonex, 0.26. Bacterial cultures showed increased coliform and enterococcal species in the rats consuming elemental diets.
๐ SIMILAR VOLUMES
The quantitative analysis of phytonadione in several elemental diet formulations at levels ranging from 90 to 150 ppb is described. After extraction with petroleum either, the sample is cleaned up by silica gel column chromatography and separated on a TLC plate; phytonadione is determined by reflect