𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Alteration of thrombine-signaling mechanism by heptachlor in human platelets

✍ Scribed by María G. Moya de Juri; Gladis Magnarelli de Potas; Ana M. Pechen de D'Angelo

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
180 KB
Volume
16
Category
Article
ISSN
1095-6670

No coin nor oath required. For personal study only.

✦ Synopsis

Heptachlor is a persistent organochlorine insecticide that has been detected in human tissues and fluids. The ability of heptachlor to interfere with platelet phosphoinositides metabolism and related signaling events stimulated by thrombin was evaluated. In vitro incubations with a concentration range of 1-100 microM heptachlor, prior to platelets activation, were performed. Experiments showed that 10 microM increased protein Kinase C (PKC) activity and phosphatidylinositolbiphosphate and phosphatidic acid phosphorylation. Simultaneously phosphatidylcholine and phosphatidylethanolamine breakdown were prevented. Similar effects were observed with HC 1 microM. However, heptachlor 100 microM increased phosphatidylinositolbiphosphate phosphorylation but reduced serine/threonine kinases activity. We propose that signal transduction steps downstream phospholipase C (PLC) are unphysiologically activated by heptachlor and facilitated by the increase in phosphatidylinositolbiphosphate, the substrate for PLC activity, thus producing an accumulation of phosphatidic acid. The elevated level of this compound itself or the transient increase in diacylglycerol produced may cause calcium mobilization and the activation of PKC. In contrast with the alterations observed in phospholipids and protein phosphorylation, no changes in aggregation properties were observed.

📜 SIMILAR VOLUMES

Microtubule inhibitors alter the secreti
Microtubule inhibitors alter the secretion of β-glucuronidase by human blood platelets: Involvement of microtubules in release reaction II
✍ Dianne M. Kenney; Francis C. Chao 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 794 KB

## Abstract The effects of the antimicrotubular drugs colchicine and vinblastine on the blood platelet release reaction were studied by measuring release of ^14^C‐5‐hydroxytryptamine (^14^C‐5‐HT, release I) and β‐glucuronidase (release II) from gel‐filtered human platelets. β‐glucuronidase release

Conditions influencing release of granul
Conditions influencing release of granule contents from human platelets in citrated plasma induced by ADP or the thrombin receptor activating peptide SFLLRN: Direct measurement of percent release of β-thromboglobulin and assessment by flow cytometry of P-selectin expression
✍ Rand, Margaret L.; Perry, Dennis W.; Packham, Marian A.; Gemmell, Cynthia H.; Ye 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 695 KB

Contrary to a recent report [Rinder et al.: Blood 82:505, 19931, aspirin does inhibit the release of a-granule contents as well as inhibiting the release of dense granule contents by human platelets during ADP-induced aggregation in citrated platelet-rich plasma (PRP). Measurements were: percent rel

Multiple mechanisms of action of ZR2002
Multiple mechanisms of action of ZR2002 in human breast cancer cells: A novel combi-molecule designed to block signaling mediated by the ERB family of oncogenes and to damage genomic DNA
✍ Fouad Brahimi; Zakaria Rachid; Qiyu Qiu; James P. McNamee; Yu-Jiang Li; Ana M. T 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 French ⚖ 276 KB 👁 2 views

## Abstract The mechanism of action of ZR2002, a chimeric amino quinazoline designed to possess mixed EGFR tyrosine kinase (TK) inhibitory and DNA targeting properties, was compared to those of ZR01, a reversible inhibitor of the same class and PD168393, a known irreversible inhibitor of EGFR. ZR20