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Alteration of the Fatty Acid Substrate Specificity of Lysophosphatidate Acyltransferase by Site-Directed Mutagenesis

โœ Scribed by Larry Zee Morand; Shilpa Patil; Mary Quasney; J.Bruce German

Book ID
115581717
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
112 KB
Volume
244
Category
Article
ISSN
0006-291X

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Alteration of the substrate range of hal
Alteration of the substrate range of haloalkane dehalogenase by site-directed mutagenesis
โœ Paul Holloway; Kyle L. Knoke; Jack T. Trevors; Hung Lee ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 58 KB ๐Ÿ‘ 1 views

We attempted to expand the range of chlorinated solvents degraded by Xanthobacter autotrophicus GJ10 to include trichloroethylene by the rational modification of the enzyme haloalkane dehalogenase. The amino acids Phe164, Asp170, Phe172 and Trp175 were individually replaced with alanine by site-dire