Alteration of cell membrane by stress waves in vitro

✍ Scribed by Shun Lee; Therese Anderson; Hong Zhang; Thomas J. Flotte; Apostolos G. Doukas

• Book ID: 104324589
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 801 KB
• Volume: 22
• Category: Article
• ISSN: 0301-5629
• DOI: 10.1016/s0301-5629(96)00149-4

✦ Synopsis

Experiments on the biological effects of laser-induced stress waves indicate that there is a transient increase in the permeability of the cell membrane. A cell viability assay ( propidhnn iodide exclusion) shows that mouse breast sarcoma cells are viable after a stress wave. The kinetics of this transient membrane permeability are measured using time-resolved fluorescence imaging. The efflux of a membrane-impermeable fluorescent probe (calcein) following the application of a 300-bar stress wave implies that there is an increase in the membrane permeability. This efIiux ceases within 80 s after a stress wave, suggesting that the membrane is no longer permeable to the fluorescent probe. Fitting the observed kinetics to a simple dlffusion model yields an average initial diffusion constant of 2.2 2 1.3 x lo-' cm2/s for mouse breast sarcoma cells following the application of a laser-induced stress wave.