Alpha-smooth-muscle actin and desmin expressions in human neuroblastoma cell lines

✍ Scribed by Tohru Sugimoto; Hisao Ueyama; Hajime Hosoi; Johji Inazawa; Taiji Kato; John T. Kemshead; C. Patrick Reynolds; Allen M. Gown; Hiroshi Mine; Tadashi Sawada

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 847 KB
• Volume: 48
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910480221

✦ Synopsis

The neural crest gives rise to a variety of tissues, including peripheral neurons, Schwann cells, melanocytes and ectomesenchymal cells, which include the smooth-muscle cells of large arteries. Cell lines derived from neuroblastoma (a neuralcrest tumor) exhibit at least 2 distinct morphological cell types, a neuroblastic phenotype (N-type) and an epitheliallike phenotype (S-type) with characteristics of substrateadhesiveness. We have analyzed I 7 human neuroblastoma cell lines using a panel of monoclonal antibodies (MAbs) against cytoskeletal proteins. Three neuroblastoma cell lines (KP-N-SI, KP-N-YN and SMS-KCN) bound an a-smoothmuscle actin antibody. In addition, one of these lines (KP-N-SI) bound anti-desmin MAbs as determined by indirect immunofluorescence. A total of 8 cloned cell lines were obtained from the above parent cell lines. These were composed of either Nor S-type cells and were confirmed to have the same neuroblastoma origin as each parent cell line by chromosomal analysis. Alpha-smooth-muscle actin and desmin were demonstrated in the S-type cloned cells by indirect immunofluorescence, as well as by 2-dimensional Western blot analysis. These results were confirmed by Northern blot analysis using a specific probe (pSHaSMA-3'UT) to human asmooth-muscle actin mRNA. These ascertain the presence of a-smooth-muscle actin and desmin in neuroblastoma cell lines. These data show that, in addition to giving rise to cells with neural, Schwann-cell and melanocyte markers, neuroblastoma can also give rise to the cells expressing rmoothmuscle cell markers.